beta-Galactosidase alpha-complementation. Overlapping sequences.
نویسندگان
چکیده
منابع مشابه
Differential expression of three alpha-galactosidase genes and a single beta-galactosidase gene from Aspergillus niger.
A gene encoding a third alpha-galactosidase (AglB) from Aspergillus niger has been cloned and sequenced. The gene consists of an open reading frame of 1,750 bp containing six introns. The gene encodes a protein of 443 amino acids which contains a eukaryotic signal sequence of 16 amino acids and seven putative N-glycosylation sites. The mature protein has a calculated molecular mass of 48,835 Da...
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The characterization of protein interactions is important to the understanding of signal transduction pathways and cellular processes. Here we describe a method utilizing β-galactosidase (βgal) complementation that can monitor protein interactions in live mammalian cells (Rossi et al. 1997, 2000; Blakely et al. 2000). In brief, the method involves expressing chimeric proteins consisting of two ...
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The unrefined fold of Escherichia coli beta-galactosidase based on a monoclinic crystal form with four independent tetramers has been reported previously. Here, we describe a new, orthorhombic form with one tetramer per asymmetric unit that has permitted refinement of the structure at 1.7 A resolution. This high-resolution analysis has confirmed the original description of the structure and rev...
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The amino acid sequence of 72 chymotryptic peptides isolated from 14C-, 3H-labeled carboxymethyl-beta-galactosidase has been determined. A variety of techniques were used in the isolation procedures including separation by solubility, size, and ion exchange and paper chromatography. These peptides contain approximatley 500 amino acids, range in size from 2 to 26 residues, and give overlaps with...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1981
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)69063-0